IGNOU MBC-03
- Bioanalytical Techniques,
Latest Solved Assignment
(January 2024 - December 2024)

PART-(A)

1. a) Define the following:

i) Buffers
ii) Parts per million (ppm)
iii) Resolving power
iv) Cryofixation
v) Sedimentation coefficient
b) Explain the principle of Beer-Lambert law.

2.a) Discuss the density gradient centrifugation.
b) Explain the working principle and basic components of florescence microscopy.

3.a) Discuss Scanning Tunneling Microscope.
b) Compare ORD and CD Spectroscopy

4. a) Write a brief account note on Laboratory safety rules.
b) Explain the following:
i) Homogenisation
ii) Freeze thaw

5. (a) How would you make the solutions of following concentration?
i) 100 ml of 0.5 M Sucrose solution
ii) 50 ml of 1 Normal solution of HCl.
b) Discuss different methods of ionization in mass spectrophotometry,

PART- (B)

6. Differentiate between:

a) Affinity chromatography and Ion exchange chromatography
b) Northern and Southern technique

7. a) What are supproting media used in electrophoresis?.
b) Discuss the genomic in situ hybridization (GISH) technique.

8. a) State are the major parts of the HPLC Liquid chromatography systems.
b) Describe SDS-PAGE and its significance in characterization of proteins.

9. (a) Define radioisotopes half-life. Write applications of radioactivity.
b) Explain scintillation counting with suitable diagram.

10. Elaborate the principle and applications of the following:

a) LC/MS
b) UPLC