1. Explain the classification of amino acids based on structure and metabolic fate.
2. Write a short note on biologically active peptides.
3. With the help of schematic diagrams explain non-mechanical methods of cell disruption.
4. Discuss the protein concentrating technique that is based on freeze drying principle.
5. Write the principles of thin layer, gelfitration and affinity chromatography. Explain their applications.
6. Give a detailed account on chromatographic technique used for the separation of volatile compounds.
7. A) Describe the working principle of electrophoresis and list its applications.
B) Explain the N-terminal protein sequencing method
8. A) Discuss how enzymes are used for generation of protein fragments?
B) Explain the working principle of mass spectrometry
9. A) Write a short note on applications of tandem mass spectrometry.
10. A) Draw Ramachandran plot and explain it importance.
B) What is NMR? List its important applications of protein structure determination.
11. Describe principles of three techniques used for protein 3-D structure analysis.
12. What is a Biological data base? Give suitable examples.
13. Give a detailed account of structural proteins with diagrams.
14. What are immune globulins? Give a short account on their classifications.
1. Describe the properties of amino acids.
2. Describe mechanical methods of cell disruption with suitable examples.
3. With the help of schematic diagram explain separation of proteins using ammonium sulphate precipitation method.
4. List four important applications and principles of each of the following: thin layer chromatography, high performance chromatography, affinity chromatography, and ion exchange chromatography.
5. Write the principle of electrophoresis. Explain Isoelectric focusing using a neatly label diagram. List its advantages.
6. Give a detailed account on Sanger’s protein sequencing method.
7. Describe the working principle of Tandem mass spectrometry and its applications.
8. Illustrate the secondary structure of proteins.
9. Write a short note on protein mis-folding diseases.
10. What is bioinformatics? Give a detailed account on importance of biological databases.
11. Describe specified functions of proteins.
12. Explain NMR principle.
13. What is immunity? Describe the structure of immunoglobulins Ig E and Ig M using.
14. Draw the structure of sarcomere. Explain sliding filament model of muscle contraction.
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